CURRICULUM VITAE. Carol R. Gardner

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1 CURRICULUM VITAE Carol R. Gardner Address: Rutgers University Ernest Mario School of Pharmacy 160 Frelinghuysen Rd. Department of Pharmacology and Toxicology Piscataway, New Jersey Phone: (732) Fax: (732) EDUCATION: Ph.D - Texas Woman's University, Denton, Texas, Radiation Biology, May M.S. - Southern Illinois University at Edwardsville, Edwardsville, Illinois, Biological Sciences, Aug B.A. - State University College at Potsdam, Potsdam, New York, Biology, Cum Laude, May PROFESSIONAL EXPERIENCE: Immunology Instructor Office of Special Academic Programs, UMDNJ-Robert Wood Johnson Medical School, Summer Associate Research Professor - Dept. of Pharmacology and Toxicology, Rutgers University, Ernest Mario School of Pharmacy, 7/98-present. Assistant Research Professor - Dept. of Pharmacology and Toxicology, Rutgers University, College of Pharmacy, 8/88-7/98. Postdoctoral Fellow - Rutgers University, College of Pharmacy, 8/85-8/88. Research and Teaching Assistant - Texas Woman's University, 8/80-5/85. Research and Teaching Assistant - Southern Illinois University, 9/78-5/80. Summer Clerkship Program in Cancer Research - Brooklyn Jewish Hospital, 5/76-8/76. TEACHING EXPERIENCE: UMDNJ-Robert Wood Johnson Medical School Immunology, Biomedical Careers Program, Summer 2006 Rutgers University - Lectures in Advanced General Pharmacology I and II, Cellular Immunology, Pathophysiology, Advanced General Toxicology I, Advanced Microbiology, Pharmacology. Co-coordinator for Advanced General Toxicology , Co-coordinator for Graduate Seminar Class Co-coordinator for Pathophysiology Texas Woman's University - Comparative Anatomy Lab, Mammalian Physiology Lab, Human Physiology Lab, Cat Anatomy Lab, Human Anatomy and Physiology Lab, Animal Biology Lab. Southern Illinois University - Anatomy and Physiology Lab, Bacteriology Lab. 1

2 UNDERGRADUATE STUDENTS SUPERVISED: Karen Stephenson - Biomedical Careers Program, Summer 1988 Parthiv Mahadevia - Independent Study, Summer 1988 Adele Wing - Brunel University Placement, Summer 1989 Sumant Ramachandra - Honors Student, 9/89-8/90 Eneya Mulagha - Biomedical Careers Program, Summer 1990 Michelle Landman - Honors Student, College of Pharmacy, 2/91-5/94 Nosheen Ahmad - Independent Study, 9/93-5/95 Muhammad Arif - Independent Study, 5/95-12/96 Shalu Madan - Spring 1996 Louis M. Allegretto - Independent Study, 1/97-5/97 Renee Epner - Independent Study, 1/97-5/97 Reena Amin - Independent Study, 6/03-present Rosanna Caroleo - Independent Study, 1/03-6/04 Nadine Pierre - Independent Study, 11/03 6/06 GRADUATE STUDENTS SUPERVISED: Lorena Bonilla - M.S., 8/99-5/00 GRANTS FUNDED: "Role of Soluble Immune Mediators in Lung Injury", American Lung Association - $ 8,000, 7/1/90-6/30/91 "Characterization of Immune Mediator Production by Lung Macrophages", BRSG funds - $4,710, 9/1/90-5/31/91 "The Role of Kupffer Cells in Drug-Induced Hepatotoxicity", BRSG funds - $5,782, 10/1/91-9/1/92 "Studies on the Role of Nonparenchymal Cells in Liver Fibrosis Induced by Carbon Tetrachloride", EOHSI Seed Money grant - $13,200, 12/1/92-11/30/93 "The Role of Nonparenchymal Cells in Tumor-Induced Liver Dysfunction", New Jersey State Commission on Cancer Research - $84,579, 6/1/93-5/31/95 ABenzene Metabolism and Toxicity in TG:AC Mice@, Exploratory Research Award, EOHSI - $14,900, 4/1/98-3/31/99. ARole of Kupffer Cells in Chemical Toxicity@, co-investigator, PHS NIH-NIHGMS - $710,000 total, $185,000 annual direct costs, 4/1/98-3/31/02. ARole of Collagens Type XII and XIV in Carbon Tetrachloride Induced Liver Fibrosis@, Exploratory Research Award, EOHSI - $15,000, 4/1/99-3/31/00. ARole of Kupffer Cells in Chemical Toxicity@, co-investigator, PHS NIH-NIHGMS - $1,000,000 total, $200,000 annual direct costs, 5/1/06-4/30/10. 2

3 PROFESSIONAL SOCIETIES AND HONORS: Graduated Cum Laude, State University College at Potsdam, New York, May 1978 New York Academy of Sciences Society for Leukocyte Biology Sigma Xi - Scientific Research Society Iota Sigma Pi - Woman's Chemistry Honor Society Microscopy Society of America Society for Toxicology UNIVERSITY SERVICE: Volunteer in Rutgers International Friendship Program Member, Library Committee - Ernest Mario School of Pharmacy, , , , Member, Ad Hoc Committee on Distilled Water System - College of Pharmacy, Member, Seminar Committee - Dept. Pharmacology and Toxicology, Member, Environmental and Occupational Health Sciences Institute present Associate Member, Joint Graduate Program in Toxicology, 1997-present. Member, EOHSI-CRESP Radiation Safety Committee Associate Director, EOHSI-CINJ Shared Flow Cytometry Resource present Member, Multicultural Affairs Committee - Ernest Mario School of Pharmacy, , Member, Self-Study Subcommittee on Faculty - Ernest Mario School of Pharmacy, 2000 Member, Joint Graduate Program in Toxicology Membership Committee Member, Admissions and Recruitment Committee - Ernest Mario School of Pharmacy, Member, Organization and Administration Self Study Committee Ernest Mario School of Pharmacy, REFEREED PUBLICATIONS (34): R.J. Laumbach, N. Fiedler, C.R. Gardner, D.L. Laskin, Z.H. Fan, J. Zhang, C.J. Weschler, P.J. Lioy, R.B. Devlin, P. Ohman-Strickland, K. Kelly-McNiel, and H.M. Kipin Nasal effects of a mixture of volatile organic compounds and their ozone oxidation products. J. Occup. Environ. Med. 47(11): B. Weinberger, N. Hanna, J.D. Laskin, D.E. Heck, C.R. Gardner, D.R. Gerecke, and D.L. Laskin Mechanisms mediating the biologic activity of synthetic proline, glycine, and hydroxyproline polypeptides in human neutrophils. Mediators Inflamm. 2005(1): L. Fakhrzadeh, J.D. Laskin, C.R. Gardner, and D.L. Laskin Superoxide dismutase-overexpressing mice are resistant to ozone-induced tissue injury and increases in nitric oxide and tumor necrosis factor-α. Am. J. Respir. Cell Mol. Biol. 30: X. Zhang, W. Li, S. Pooyan, Y. Su, C.R. Gardner, M.J. Leibowitz, S. Stein, and P. Sinko Quantitative assessment of the cell penetrating properties of RI-Tat9: Evidence for a cell type-specific barrier at the plasma membrane of epithelial cells. Mol. Pharmaceut. 1: H. Chiu, C.R. Gardner, D.M. Dambach, S.K. Durham, J.A. Brittingham, J.D. Laskin, and D.L. Laskin. 3

4 2003. Role of tumor necrosis factor receptor 1 (p55) in hepatocyte proliferation during acetaminopheninduced toxicity in mice. Toxicol. Appl. Pharmacol. 193: C.R. Gardner, J.D. Laskin, D.M. Dambach, H. Chiu, S.K Durham, P. Zhou, M. Bruno, D.R. Gerecke, M.K. Gordon, and D.L. Laskin Exaggerated hepatotoxicity of acetaminophen in mice lacking tumor necrosis factor receptor-1. Potential role of inflammatory mediators. Toxicol. Appl. Pharmacol. 192: H. Chiu, C.R. Gardner, D.M. Dambach, S.K. Durham, J.A. Brittingham, J.D. Laskin and D.L. Laskin Role of p55 tumor necrosis factor receptor 1 in acetaminophen-induced antioxidant defense. Am. J. Physiol. Gastrointest. Liver Physiol. 285:G959-G966. C.R. Gardner, J.D. Laskin, D.M. Dambach, M.Sacco, S.K. Durham, M.K. Bruno, S.D. Cohen, M.K. Gordon, D.R. Gerecke, P. Zhou and D.L. Laskin Reduced hepatotoxicity of acetaminophen in mice lacking inducible nitric oxide synthase: Potential role of tumor necrosis factor-α and interleukin-10. Toxicol. Appl. Pharmacol.184(1): B. Billack, D.E. Heck, T.M. Mariano, C.R. Gardner, R. Sur, D.L. Laskin, and J.D. Laskin Induction of cyclooxygenase-2 by heat shock protein 60 in macrophages and endothelial cells. Am. J. Physiol. Cell Physiol. 283:C1267-C1277. V.R. Sunil, A.J. Conner, N. Lavnikova, C.R. Gardner, J.D. Laskin, and D.L. Laskin Acute endotoxemia prolongs the survival of rat lung neutrophils in response to 12-O-tetradecanoyl-phorbol 13- acetate. J. Cell. Physiol. 190: B. Weinberger, D.L. Laskin, T.M. Mariano, V.R. Sunil, C.J. DeCoste, D.E. Heck, C.R. Gardner, and J.D. Laskin Mechanisms underlying reduced responsiveness of neonatal neutrophils to distinct chemoattractants. J. Leukoc. Biol. 70 (6): J.D. Laskin, D.E. Heck, C.R. Gardner, and D.L. Laskin Prooxidant and antioxidant functions of nitric oxide in liver toxicity. Antiox. Redox Signal. 3(2): B. Billack, D.E. Heck, M. Porterfield, R.P. Malchow, P. Smith, C.R. Gardner, D.L. Laskin, and J.D. Laskin Minimal amidine structure for inhibition of nitric oxide biosynthesis. Biochem. Pharmacol. 61(12): T. Thomas, S. Balabhadrapathruni, C.R. Gardner, J. Hong, C.A. Faaland, and T.J. Thomas Effects of epidermal growth factor on MDA-MB-468 breast cancer cells: Alterations in polyamine biosynthesis and the expression of p21/cip1/waf1. J. Cell. Physiol. 179: N. Ahmad, C.R. Gardner, E. Yurkow and D.L. Laskin Inhibition of macrophages with gadolinium chloride alters ICAM-1, MAC-1 and LFA-1 expression in the liver during acute endotoxemia. Hepatology 29(3): B. Weinberger, L. Fakhrzadeh, D.E. Heck, J.D. Laskin, C.R. Gardner, and D.L. Laskin Inhaled nitric oxide primes lung macrophages to produce reactive oxygen and nitrogen intermediates. Am. J. Resp. Crit. Care Med. 158(3): C.R. Gardner, D.E. Heck, C.S. Yang, P.E. Thomas, X.-J. Zhang, G.L. DeGeorge, J.D. Laskin, and D.L. Laskin Role of nitric oxide in acetaminophen-induced hepatotoxicity in the rat. Hepatology 4

5 27(3): K.J. Pendino, C.R. Gardner, R.L. Shuler, J.D. Laskin, S.K. Durham, D. Barton, S.T. Ohnishi, T. Ohnishi, and D.L. Laskin Inhibition of ozone-induced nitric oxide synthase expression in the lung by endotoxin. Am. J. Resp. Cell Molec. Biol. 14: K.J. Pendino, C.R. Gardner, S. Quinones, and D.L. Laskin Stimulation of nitric oxide production in rat lung lavage cells by anti-mac-1ß antibody: Effects of ozone inhalation. Am. J. Resp. Cell Molec. Biol. 14: D.L. Laskin, C.R. Gardner, V. Price, and D.J. Jollow Inhibition of macrophage functioning abrogates the acute hepatotoxicity of acetaminophen. Hepatology 21: C.R. Gardner, J.D. Laskin, and D.L. Laskin Distinct biochemical responses of hepatic macrophages and endothelial cells to platelet-activating factor during endotoxemia. J. Leukoc. Biol. 57: R.L. Shuler, D.L. Laskin, C.R. Gardner, L.S. Feder, and J.D. Laskin Lymphocyte-mediated nitric oxide production by rat endothelial cells. J. Leukoc. Biol. 57: T.M. Wizemann, C.R. Gardner, J.D. Laskin, S. Quinones, S.K. Durham, N.L. Goller, S.T. Ohnishi, and D.L. Laskin Production of nitric oxide and peroxynitrite in the lung during acute endotoxemia. J. Leukoc. Biol. 56: D.L. Laskin, D.E. Heck, C.R. Gardner, L.S. Feder, and J.D. Laskin Distinct regulation of nitric oxide production in hepatic macrophages and endothelial cells following acute endotoxemia. J. Leukoc. Biol. 56: S. Hwang, C.A. Lopez, D.E. Heck, C.R. Gardner, D.L. Laskin, J.D. Laskin, and D.T. Denhardt Osteopontin inhibits induction of nitric oxide synthase gene expression by inflammatory mediators in mouse kidney epithelial cells. J. Biol. Chem. 269: K.J. Pendino, C.R. Gardner, J.D. Laskin, and D.L. Laskin Induction of functionally active platelet activating factor receptors in rat alveolar macrophages. J. Biol. Chem. 268: C.R. Gardner, J.D. Laskin, and D.L. Laskin Platelet activating factor-induced calcium mobilization and oxidative metabolism in hepatic macrophages and endothelial cells. J. Leukoc. Biol. 53: D.E. Heck, D.L. Laskin, C.R. Gardner, and J.D. Laskin Epidermal growth factor suppresses nitric oxide and hydrogen peroxide production by keratinocytes. J. Biol. Chem. 267: C.R. Gardner, A.J. Wasserman, and D.L. Laskin Liver macrophage-mediated cytotoxicity of mastocytoma cells involves phagocytosis of tumor targets. Hepatology 14(2): J.D. Laskin, A. Dokidis, C.R. Gardner, and D.L. Laskin Changes in sulfated proteoglycan production following activation of rat liver macrophages. Hepatology 14(2): J.J. Reiners, Jr., A.R. Cantu, A. Pavone, S.C. Smith, C.R. Gardner, and D.L. Laskin Fluorescent assay for per cell estimation of cytochrome P-450 dependent monooxygenase activity in keritinocyte 5

6 suspensions and cultures. Anal. Biochem. 188: C.R. Gardner, A.J. Wasserman, and D.L. Laskin Differential sensitivity of tumor targets to liver macrophage mediated cytotoxicty. Cancer Res. 47: D.L. Laskin, C.R. Gardner, and J.D. Laskin Induction of chemotaxis in mouse peritoneal macrophages by activators of protein kinase C. J. Leukoc. Biol. 41(6): J.H. Lucas, G.W. Gross, D.G. Emery and C.R. Gardner Neuronal survival or death after dendrite transection close to the perikaryon: Correlation with electrophysiologic, morphologic and ultrastructural changes. Central Nervous System Trauma 2(4): BOOK CHAPTERS (18): C.R. Gardner and D.L. Laskin Sinusoidal Cells in Liver Injury and Repair. Hepatotoxicity: From genomics to in-vitro and in-vivo models, S.C. Sahu, ed., The Wiley Co., London. (In press). D.L. Laskin and C.R. Gardner Role of sinusoidal cells and inflammatory macrophages in hepatotoxicity. Drug-Induced Liver Disease, 2 nd ed., N. Kaplowitz and L. DeLeve, eds., Marcel Dekker, NY. pp D.L. Laskin, C.R. Gardner, D.R. Gerecke, and J.D. Laskin Ozone-induced lung injury: role of macrophages and inflammatory mediators. Reactive Oxygen/Nitrogen Species: Lung Injury and Disease, V. Vallyathan, X. Shi, and V. Castranova, eds., Marcel Dekker, NY. pp D.L. Laskin and C.R. Gardner Role of sinusoidal cells and inflammatory macrophages in hepatotoxicity. Drug-Induced Liver Disease, N. Kaplowitz and L. DeLeve, eds., Marcel Dekker, NY. pp C.R. Gardner, D.E. Heck, H. Chiu, J.D. Laskin, S.K. Durham, and D.L. Laskin Decreased hepatotoxicity of acetaminophen in mice lacking inducible nitric oxide synthase. Cells of the Hepatic Sinusoid, Vol. 7, E. Wisse, D.L. Knook, R. DeZanger, and R. Fraser, eds., Kupffer Cell Foundation, Leiden. pp C.R. Gardner and D.L. Laskin Protective and pathologic roles of nitric oxide in tissue injury. Cellular and Molecular Biology of Nitric Oxide, J.D. Laskin and D.L. Laskin, eds, Marcel Dekker, NY. pp D.L. Laskin and C.R. Gardner The role of nonparenchymal cells and inflammatory macrophages in hepatotoxicity. Toxicology of the Liver, 2 nd Edition, G.L. Plaa and W.R. Hewitt, eds., Raven Press, NY. pp C.R. Gardner, S.K. Durham, D. Barton, D.E. Heck, J.D. Laskin, and D.L. Laskin Role of nitric oxide in hepatotoxicity. Cells of the Hepatic Sinusoid, Vol. 6, E. Wisse, D.L. Knook, and C. Balabaud, eds., Kupffer Cell Foundation, Leiden. pp D.L. Laskin, D.E. Heck, C.R. Gardner, S.K. Durham, N.L. Goller, S.T. Ohnishi, and J.D. Laskin Enhanced production of nitric oxide by hepatic macrophages (MP), endothelial cells (EC) and fat storing cells (FSC) following acute endotoxemia. Cells of the Hepatic Sinusoid, Vol. 5, E. Wisse, D.L. Knook, and K. Wake, eds., Kupffer Cell Foundation, Leiden. pp

7 C.R. Gardner, J.D. Laskin, and D.L. Laskin Platelet activating factor (PAF) induced activation of hepatic macrophages and endothelial cells: Role of intracellular calcium. Cells of the Hepatic Sinusoid, Vol. 4, E. Wisse, D.L. Knook, and R.S. McCuskey, eds., Kupffer Cell Foundation, Leiden. pp D.L. Laskin, D.E. Heck, L.S. Feder, C.R. Gardner, and J.D. Laskin Regulation of nitric oxide production by hepatic macrophages and endothelial cells. Cells of the Hepatic Sinusoid, Vol. 4, D.L. Knook, and E. Wisse, eds., Kupffer Cell Foundation, Leiden. pp C.R. Gardner, D.E. Heck, L.S. Feder, T.W. Mc Closkey, J.D. Laskin, and D.L. Laskin Differential regulation of reactive nitrogen and reactive oxygen intermediate production by hepatic macrophages and endothelial cells. The Molecular Basis of Oxidative Damage by Leukocytes, A. Jesaitis, and E.A. Dratz, eds., CRC Press, Boca Raton. pp C.R. Gardner, J.D. Laskin, C.A. Faaland and D.L. Laskin Role of protein kinases in liver macrophage function. Cells of the Hepatic Sinusoid, Vol. 3, E. Wisse, D.L. Knook, and R.S. McCuskey, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp D.L. Laskin, L.S. Feder, T.W. Mc Closkey, and C.R. Gardner Parenchymal and nonparenchymal cell (NPC) interactions in hepatotoxicity. Cells of the Hepatic Sinusoid, Vol. 3, E. Wisse, D.L. Knook, and R.S. McCuskey, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp T.W. Mc Closkey, J.A. Todaro, L.S. Feder, A.J. Wasserman, C.R. Gardner, and D.L. Laskin Activation of liver macrophages (MP) and endothelial cells (EC) following lipopolysaccharide (LPS) treatment of rats. Cells of the Hepatic Sinusoid, Vol. 3, E. Wisse, D.L. Knook, and R.S. McCuskey, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp C.R. Gardner, A.J. Wasserman, and D.L. Laskin Phagocytosis of tumor cells by activated liver macrophages. Cells of the Hepatic Sinusoid, Vol. 2, E. Wisse, D.L. Knook, and K. Decker, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp F.M. Robertson, T.W. Mc Closkey, T.M. Obersyzyn, C.R. Gardner, and D.L. Laskin Lymphokine induced modulation of accessory cell function and inflammatory potential of Kupffer cells. Cells of the Hepatic Sinusoid, Vol. 2, E. Wisse, D.L. Knook, and K. Decker, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp J.D. Laskin, A. Dokidis, C.R. Gardner, F.M. Robertson, and D.L. Laskin Role of sulfated proteoglycans in Kupffer cell function. Cells of the Hepatic Sinusoid, Vol. 2, E. Wisse, D.L. Knook, and K. Decker, eds., Kupffer Cell Foundation, Rijswijk, Netherlands. pp

8 ABSTRACTS (92): C.R. Gardner, J.P. Gray, J. Dubois, J.D. Laskin, and D.L. Laskin Role of caveolin-1 and endothelial nitric oxide synthase in acetaminophen-induced hepatotoxicity. The Toxicologist 96(S1):177. (Abstract no. 856). C.R. Gardner, J.P. Gray, J. Dubois, J.D. Laskin, and D.L. Laskin Reduced hepatotoxicity of acetaminophen in caveolin-1 (Cav-1) knockout mice is associated with increased tissue repair. The Toxicologist 90(S1):205. (Abstract no. 1007). C.R. Gardner, H. Chiu, J.D. Laskin, and D.L. Laskin Acetaminophen-induced upregulation of PI- 3K and NF-κB in mouse liver: Regulation by TNFα and caveolin-1. The Toxicologist 84(S1):215. (Abstract no. 1054). C.R. Gardner, L. Chen, J.D. Laskin, and D.L. Laskin Role of Toll-like receptor-4 (TLR-4) in acetaminophen (AA)-induced hepatotoxicity. The Toxicologist 78(S1):105. (Abstract no. 511). C.R. Gardner, N. Hanna, B. Weinberger, L. Bonifacio, J. Schak, S. Graboski, and D.L. Laskin Regulation of cyclooxygenase-2 (COX-2) expression and prostaglandin E 2 (PGE 2 ) production by interferon gamma in human placenta and isolated placental macrophages. J. Leukoc. Biol. (Supplement): 19. Abstract 18. D.L. Laskin, C.R. Gardner, D.E. Heck, and J.D. Laskin Hepatic macrophages, nitric oxide and cytokines in drug-induced liver injury. Drug Metabol. Rev. 35 (S2), 12th North American Meeting of the International Society for the Study of Xenobiotics, Providence, Rhode Island, Oct , R.J. Laumbach, N. Fiedler, D.L. Laskin, C.R. Gardner, J. Zhang, T. Fan, K. Kelly-McNeil, P. Lioy, P. Ohman-Strickland, and H.M. Kipen Heat shock protein (HSP) 60: A sensitive biomarker of nasal inflammation? J. Occup. Environ. Med. 45(7): L. Fakhrzadeh, T. DeSimone, C.R. Gardner, V.R. Sunil, J.D. Laskin, D.E. Heck, and D.L. Laskin Regulation of nitric oxide and PGE 2 production by alveolar macrophages following ozone inhalation. Amer. J. Resp. Crit. Care Med. 167:A481. R.J. Laumbach, N. Fiedler, D.L. Laskin, C.R. Gardner, J. Zhang, P. Lioy, T. Fan, M.S. Soukup, R.B. Devlin, K. Kelly-McNeil, P. Ohman-Strickland, and H.M. Kipen Nasal effects of VOCs and ozone. Amer. J. Resp. Crit. Care Med. 167:A972. D.E. Heck, C.R. Gardner, P. Zhou, D. Gerecke, M.A. Gordon, and J.D. Laskin Regulation of STAT-mediated signaling by the potent tumor promoter 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in endothelial cells. Proc. Am. Assoc. Cancer Res. 44:473.(Abstract no. 2074). N. Fiedler, J. Zhang, T. Fan, K. Kelly-McNeil, P. Lioy, C.R. Gardner, and H. Kipen Health effects of a volatile organic mixture with and without ozone. Indoor Air th International Conference on Indoor Air Quality and Climate, June 30 - July 5, 2002, Monterey, CA. D. Heck, P. Zhou, J. Laskin and C. Gardner ,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces functional activation of the gamma-interferon activated sequence in rat hepatic endothelial cells. The Toxicologist 66(S1):8. (Abstract no. 39). 8

9 H. Chiu, C.R. Gardner, S.G. Ricketts, J.D. Laskin, and D.L. Laskin Increased hepatotoxicity of acetaminophen in tumor necrosis factor alpha (TNF) R1 (p55) knockout mice is associated with decreased activator protein-1 (AP-1) activation. The Toxicologist 66(S1):279. (Abstract no. 1367). L.F. Fakhrzadeh, K. Lee, S.G. Ricketts, C.R. Gardner, V.R. Sunil, J.D. Laskin, and D.L. Laskin Ozone toxicity is associated with upregulation of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (PKB) in lung macrophages: Role of caveolin-1. The Toxicologist 66(S1):196. (Abstract no. 957a). L.F. Fakhrzadeh, C.R. Gardner, V.R. Sunil, J.D. Laskin, D.L. Laskin Role of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (PKB) in regulating nitric oxide production in the lung following ozone inhalation. Am. J. Respir. Crit. Care Med. 163(5):A208. V.R. Sunil, C.R. Gardner, J.D. Laskin, and D.L. Laskin Prolonged survival of activated neutrophils (PMN) in the lung during acute endotoxemia is mediated by NF-κB. Am. J. Respir. Crit. Care Med. 161(3):A513. B. Weinberger, C.R. Gardner, D.E. Heck, J.D. Laskin, and D.L. Laskin Mechanisms underlying neutrophil chemotactic activity of collagen-like polypeptides. Pediatric Academic Societies Meeting, May 2000, Boston, MA. C.R. Gardner, D.M. Dambach, S.K. Durham, S.D. Cohen, M.K. Bruno, A.L. Salzman, G.J. Southan, and D.L. Laskin Role of peroxynitrite (PN) in acetaminophen (AA)-induced hepatotoxicity. The Toxicologist 54(1):41. (Abstract no. 196) L. Fakhrzadeh, C.R. Gardner, A.L. Salzman, G.J. Southan, J.D. Laskin and D.L. Laskin Peroxynitrite contributes to ozone-induced lung injury. The Toxicologist 54(1):167. (Abstract no. 784) B. Weinberger, D.E. Heck, T.M. Mariano, E. Yurkow, C.R.Gardner, D.L. Laskin, and J.D. Laskin Mechanisms underlying the developmental regulation of neutrophil chemotaxis. Pediatric Res. 45 (4, part 2):265A. B. Weinberger, L. Fakhrzadeh, C.R. Gardner, D.E. Heck, J.D. Laskin, and D.L. Laskin Mechanisms underlying the pulmonary toxicity of inhaled nitric oxide. Pediatric Res. 45 (4, part 2):326A. L. Fakhrzadeh, J.D. Laskin, C.R. Gardner, and D.L. Laskin Role of peroxynitrite in ozoneinduced lung injury. Am. J. Respir. Crit. Care Med. 159 (3, part 2):A272. B. Weinberger, D.L. Laskin, D.E. Heck, L. Fakhrzadeh, C.R. Gardner and J.D. Laskin Reduced toxicity of inhaled nitric oxide in mice lacking the gene for inducible nitric oxide synthase and in mice overexpressing superoxide dismutase. Am. J. Respir. Crit. Care Med. 159 (3, part 2):A272. S.S. Bentivegna, C.R. Gardner, R. Snyder, J.D. Laskin, and D.L. Laskin Role of nitric oxide and tumor necrosis factor-α in benzene induced bone marrow suppression. Am. Assoc. Cancer Res. Proceedings 40:742. (Abstract no. 4899). V.R. Sunil, D.E. Heck, C.R. Gardner, J.D. Laskin, and D.L. Laskin Mechanisms regulating increased survival of adherent vascular lung neutrophils during acute endotoxemia. Am. Assoc. Cancer Res. Proceedings 40:323. (Abstract no. 2141). 9

10 D.M. Dambach, C.R. Gardner, H. Chiu, M.W. Marino, S.K. Durham, and D.L. Laskin Increased sensitivity of tumor necrosis factor-α (TNF-α) knockout mice to acetaminophen (AA) is mediated by nitric oxide (NO). The Toxicologist 48 (S1):257. (Abstract no. 1215). C.R. Gardner, D.E. Heck, H. Chiu, J.D. Laskin, S.K. Durham, and D.L. Laskin Decreased hepatotoxicity of acetaminophen in mice lacking inducible nitric oxide synthase. Eighth International Symposium on Cell of the Hepatic Sinusoid, Christchurch, New Zealand, Sept J.D. Laskin, C.R. Gardner, D.E. Heck, H. Chiu, S.K. Durham, and D.L. Laskin Mice lacking inducible nitric oxide synthase are protected from acetaminophen-induced hepatotoxicity. Toxicol. Lett. 95 (Suppl. 1):113. (Abstract no. P2C80). B.I. Weinberger, L. Fakhrzadeh, C.R. Gardner, D.E. Heck, J.D. Laskin, and D.L. Laskin Mice lacking inducible nitric oxide synthase exhibit reduced responsiveness to the irritant-inducing effects of inhaled nitric oxide. Pediatric Res. 43(4, pt. 2):302A. (Abstract no. 1772). D.E. Heck, C.R. Gardner, D.L. Laskin, and J.D. Laskin Regulation of nitric oxide production and apoptosis in human mammary carcinoma cells by 17-[beta]-estradiol. Proc. Amer. Assoc. Cancer Res. 39:113. (Abstract no. 770). N. Ahmad, C.R. Gardner, and D.L. Laskin Inhibition of macrophages with gadolinium chloride alters ICAM-1, MAC-1 and LFA-1 expression in the liver during acute endotoxemia. Toxicological Sci. 42(1S): 367. (Abstract no. 1806). C.R. Gardner, D.E. Heck, H. Chiu, J.D. Laskin, S.K. Durham, and D.L. Laskin Decreased hepatotoxicity of acetaminophen in mice lacking inducible nitric oxide synthase. Toxicological Sci. 42(1S):363. (Abstract no. 1790). D.E. Heck, B. Weinberger, C.R. Gardner, L. Fakhrzadeh, J.D. Laskin, and D.L. Laskin Cell cycle regulation of alveolar macrophage NF-kappa B activity. Am. J. Resp. Crit. Care Med. 155:A700. (Abstract no. C78). D.L. Laskin, D.E. Heck, Y. Guo, C.R. Gardner, and J.D. Laskin Acute endotoxemia is associated with increased nitric oxide production by Type II cells and activation of NF-κB. Am. J. Resp. Crit. Care Med. 155:A616. (Abstract no. C43). J.D. Laskin, B. Weinberger, L. Fakhrzadeh, D.E. Heck, C.R. Gardner, and D.L. Laskin Inhalation of nitric oxide is associated with functional activation of alveolar macrophages. Am. J. Resp. Crit. Care Med. 155:A959. (Abstract no. D80). B. Weinberger, C.R. Gardner, L. Fakhrzadeh, J.D. Laskin, D.L. Laskin and D.E. Heck Upregulation of alveolar macrophages following inhalation of nitric oxide. J. Invest. Med. 45:193A. B. Weinberger, C.R. Gardner, L. Fakhrzadeh, J.D. Laskin, D.L. Laskin, and D.E. Heck Inhibition of NF-κB nuclear apoptosis and expression of BCL-2 in macrophages. Pediatric Res. 41 (4):13A. (Abstract no. 62). B. Weinberger, C.R. Gardner, L. Fakhrzadeh, D.E. Heck, J.D. Laskin, and D.L. Laskin Increased 10

11 peroxynitrite production by alveolar macrophages (AM) following inhalation of nitric oxide (NO). Pediatric Res. 41 (4):273A. (Abstract no. 1621). C.R. Gardner, J.D. Laskin, D.E. Heck, P.E. Thomas, C.S. Yang and D.L. Laskin The role of nitric oxide in acetaminophen-induced hepatotoxicity. Hepatology 24 (4, part 2):335A. (Abstract no. 836). N. Ahmad, C.R. Gardner, and D.L. Laskin Differential induction of ICAM-1, MAC-1 and LFA-1 on liver macrophages, endothelial cells and hepatocytes following acute endotoxemia. Hepatology 24 (4, part 2):328A. (Abstract no. 806). Z. Zhou, J.D. Laskin, C.R. Gardner, Y. Jin, D.L. Laskin, and D.E. Heck Regulation of endothelial cell nitric oxide in antitumor host defense. Proc. Amer. Assoc. Cancer Res. 37:234. (Abstract no. 1598). B. Weinberger, C.R. Gardner, J.D. Laskin, and D.L. Laskin Differential expression of a unique neutrophil chemotactic antigen in adults and neonates. Pediatric Res. 39(2):292A. (Abstract no. 1736). B. Weinberger, D. Laskin, C.R. Gardner, L. Fakhrzadeh, and D. Heck Regulation of macrophage NF-κB activation during the cell cycle. Pediatric Research 39(2):15A. (Abstract no. 75). B. Weinberger, D. Laskin, C.R. Gardner, and E. Yurkow Phagocytosis in term and preterm cord blood monocytes and granulocytes. Pediatric Res. 39(2):292A. (Abstract no. 1737). N. Ahmad, C.R. Gardner, and D.L. Laskin Stimulation of nitric oxide production in hepatic macrophages (MP) and endothelial cells (EC) by cell adhesion molecules. Effects of acute endotoxemia. Hepatology 22(4, part 2):361A. (Abstract no. 1018). L. Morio, D.E. Heck, J.D. Laskin, C.R. Gardner, and D.L. Laskin Effects of long term alcohol administration on nitric oxide production by hepatic macrophages and endothelial cells. Hepatology 22(4, part 2):459A. (Abstract no. 1412). D.L. Laskin, K.J. Pendino, T.M. Wizemann, C.R. Gardner, S.K. Durham, and J.D. Laskin Induction of nitric oxide synthase gene expression in the lung following acute endotoxemia. Proc. American Thoracic Society/American Lung Assoc. International Conference, Seattle, WA, May (Abstract no ). B. Weinberger, C.R. Gardner, and D.L. Laskin Downregulation of inducible nitric oxide synthase (inos) in mouse alveolar macrophages (AM) following inhalation of nitric oxide (NO). Pediatric Res. 37(4, part 2):356A. (Abstract no. 2117). C.R. Gardner, D. Barton, N. Goller, S. Durham, and D.L. Laskin Role of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in acetaminophen hepatotoxicity. The Toxicologist 15(1):136. (Abstract no. 732). C.R. Gardner, T.M. Wizemann, S. Durham, J.D. Laskin and D.L. Laskin Acute endotoxemia induces lung macrophage (MP) production of nitric oxide (NO) and peroxynitrite. J. Leukoc. Biol. 56 (Suppl. 1994):29. (Abstract no. 74). D.E. Heck, D.L. Laskin, C.R. Gardner, and J.D. Laskin Regulation of interferon-stimulated transcription factors (STAT proteins) in alveolar epithelial cells by endotoxin. J. Leukoc. Biol. 56 (Suppl. 11

12 1994):23. (Abstract no. 39). D.L. Laskin, D.E. Heck, C.R. Gardner, S.K. Durham, N.L. Goller, S.T. Ohnishi, and J.D. Laskin Enhanced production of nitric oxide by hepatic macrophages, endothelial cells and fat storing cells following acute endotoxemia. Seventh International Symposium on Cell of the Hepatic Sinusoid, Kyoto, Japan, Sept C.R. Gardner, L.A. Morio, and D.L. Laskin Inhibition of acetaminophen hepatotoxicity by gadolinium chloride (GdCl 3 ) is associated with decreased tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) production. The Toxicologist 14:201. (Abstract no. 748). J.D. Laskin, D.L. Laskin, C.R. Gardner, and D.E. Heck Formation of peroxynitrite following treatment of keratinocytes with ultraviolet light: A cellular consequence of nitric oxide production in the skin. The Toxicologist 14:200. (Abstract no. 742). D.L. Laskin, C.R. Gardner, J.K. Maurer, and K.E. Driscoll Kupffer cell (KC)-derived tumor necrosis factor-alpha (TNF) as a mediator of hepatotoxicity. The Toxicologist 13:427. (Abstract no. 1687). D.E. Heck, D.L. Laskin, C.R. Gardner, and J.D. Laskin Role of nitric oxide in chemical induced skin injury. The Toxicologist 13:183. (Abstract no. 662). C.R. Gardner, J.D. Laskin, and D.L. Laskin Platelet activating factor (PAF)-induced alterations in intracellular ph in hepatic macrophages (MP). J. Leukoc. Biol. 52 (Suppl. 3):17. (Abstract no. 23). K.J. Pendino, C.R. Gardner, and D.L. Laskin Evidence that platelet activating factor (PAF) mediates ozone-induced alveolar macrophage activation. J. Leukoc. Biol. 52 (Suppl. 3):16. (Abstract no. 22). J.D. Laskin, D.E. Heck, C.R. Gardner, and D.L. Laskin Role of nitric oxide in the control of wound healing. J. Leukoc. Biol. 52 (Suppl. 3):25. (Abstract no. 76). L.S. Feder, D.E. Heck, C.R. Gardner, J.D. Laskin, and D.L. Laskin. Differential production of nitric oxide in hepatic macrophages (MP) and endothelial cells (EC) isolated from untreated and lipopolysaccharide (LPS)-treated rats. J. Leukoc. Biol. 52 (Suppl. 3):16. (Abstract no. 21). C.R. Gardner, J.D. Laskin, and D.L. Laskin Platelet activating factor (PAF) induced activation of hepatic macrophages and endothelial cells: Role of intracellular calcium. Sixth International Symposium on Cells of the Hepatic Sinusoid, Antwerp, Belgium, Aug K. Pendino, C. Punjabi, N. Lavnikova, C.R. Gardner, J. Laskin, and D.Laskin Inhalation of ozone stimulates nitric oxdes production by pulmonary alveolar and interstitial macrophages. American Thoracic Society/American Lung Association International Conference, May 17-20, Miami Beach, Florida. p. A650 (Abstract no. 102). D.L. Laskin, C.R. Gardner, J.A. Todaro, V. Price, and D. Jollow Inhibition of macrophage function ameliorates acetaminophen hepatotoxicity. The Toxicologist 12:290 (Abstract no. 1119). K. Pendino, C. Punjabi, C.R. Gardner, J. Laskin, and D. Laskin Effects of ozone inhalation on rat alveolar macrophage (MP) production of reactive nitrogen and oxygen intermediates. The Toxicologist 12:291 (Abstract no. 1122). 12

13 D.L. Laskin, C.R. Gardner, D.E. Heck, and J.D. Laskin Gamma interferon potentiates nitric oxide production by lipopolysaccharide (LPS) in rat hepatic macrophages (MP) and endothelial cells (EC). Cytokine 3: 463 (Abstract no. 84). C.R. Gardner, J.D. Laskin, and D.L. Laskin Induction of calcium mobilization in lipopolysaccharide (LPS)-activated liver macrophages (MP) and endothelial cells (EC) by platelet activating factor (PAF). J. Leukoc. Biol. 50 (Suppl. 2): 76 (Abstract no. 227). R.E. Petroski, V.Q. Jenab, C.R. Gardner, and H.M. Geller Assessment of neuronal adhesion/survival in culture is facilitated by labelling neurons with fluorescein diacetate. Soc. Neurosci. Abstr. 17: 373 (Abstract no ). D.L. Laskin, C.R. Gardner, L.S. Feder, D. Heck, and J.D. Laskin Nitric oxide derived from nonparenchymal liver cells (NPC) as a mediator of hepatotoxicity. The Toxicologist 11:170 (Abstract no. 605). C.R. Gardner, L.S. Feder, D.E. Heck, T.W. Mc Closkey, J.D. Laskin, and D.L. Laskin Differential regulation of reactive nitrogen and reactive oxygen intermediate production by hepatic macrophages (MP) and endothelial cells (EC). J. Cell. Biochem. Suppl. 15C:224 (Abstract no. CC 212). C.R. Gardner, J.D. Laskin, C.A. Faaland, and D.L. Laskin Role of protein kinases in liver macrophage function. J. Leukoc. Biol. 48 (Suppl. 1):101 (Abstract no. 289). C.R. Gardner, J.D. Laskin, and D.L. Laskin Role of protein kinases in liver macrophage function. Fifth International Symposium on Cells of the Hepatic Sinusoid, Tucson, Arizona, Aug D.L. Laskin, L.S. Feder, T.W. Mc Closkey, and C.R. Gardner Parenchymal and nonparenchymal cell (NPC) interactions in hepatotoxicity. Fifth International Symposium on Cells of the Hepatic Sinusoid, Tucson, Arizona, Aug T.W. Mc Closkey, J.A. Todaro, L.S. Feder, A.J. Wasserman, C.R. Gardner, and D.L. Laskin Activation of liver macrophages (MP) and endothelial cells (EC) following lipopolysaccharide (LPS) treatment of rats. Fifth International Symposium on Cells of the Hepatic Sinusoid, Tucson, Arizona, Aug C.R. Gardner, A.J. Wasserman, and D.L. Laskin Novel mechanisms of tumor cell killing by activated liver macrophages (MP). Proc. Amer. Assoc. Cancer Res. 31:243. (Abstract no. 1438). D.L. Laskin, C.R. Gardner and S. Ji Analysis of Kupffer cell (KC) function using the isolated perfused rat liver. The Toxicologist 10:59. (Abstract no. 236). D.L. Laskin, J.D. Laskin, P. Lapinskas, L. Feder, and C.R. Gardner Tissue heterogeneity in the production of tumor necrosis factor (TNF) by rat macrophages. Cytokine 1(1):110. (Abstract no. 227). C.R. Gardner, P.J. Lapinskas, L.S. Feder, and D.L. Laskin Differential production of tumor necrosis factor by rat liver, lung and peritoneal macrophages. J. Leukoc. Biol. 46(4):340. (Abstract no. 185). 13

14 C.R. Gardner, J.D. Laskin, and D.L. Laskin The potential role of protein kinase C in macrophage activation. Biology of Cellular Transducing Signals '89, IXth International Washington Spring Symposium. Washington, DC, May D.L. Laskin, A.J. Wasserman, and C.R. Gardner Modulation of chemically-induced hepatotoxicity by altering liver macrophage function. The Toxicologist 9(1):15. (Abstract no. 57). C.R. Gardner, T.W. Mc Closkey, and D.L. Laskin Gamma interferon enhanced cytotoxicity by rat liver macrophages is associated with depressed phagocytosis. J. Leukoc. Biol. 44(4):282. (Abstract no. 96). D.L. Laskin, C.R. Gardner, A.M. Pilaro, and J.D. Laskin Role of protein kinase C in the activation of liver macrophages. J. Leukoc. Biol. 44(4): (Abstract no. 69). T.W. Mc Closkey, C.R. Gardner, F.M. Robertson, and D.L. Laskin Decreased expression of immune associated (Ia) antigen and production of tumor necrosis factor by lipopolysaccharide activated liver macrophages. J. Leukoc. Biol. 44(4):295. (Abstract no. 149). A. Dokidis, C.R. Gardner, F.M. Robertson, D.L. Laskin, and J.D. Laskin Expression of chondroditin sulfates in activated rat liver macrophages. J. Leukoc. Biol. 44(4): (Abstract no. 161). C.R. Gardner, A. Dokidis, J.D. Laskin, and D.L. Laskin Alterations in sulfated proteoglycan biosynthesis in rat liver macrophages (Mφ) following in vivo activation with lipopolysaccharide (LPS). J. Leukoc. Biol. 42(4):344. (Abstract no. 48). A.J. Wasserman, C.R. Gardner, and D.L. Laskin Phagocytosis of tumor cells by activated liver macrophages. EMSA Proc. 45: C.R. Gardner and D.L. Laskin Differential sensitivity of tumor targets to resident and activated liver macrophages. J. Leukoc. Biol. 40(3):283. (Abstract no. 88). C.R. Gardner, J.D. Laskin and D.L. Laskin Evidence that protein kinase C mediates chemotaxis in mouse peritoneal macrophages. Proc. Amer. Assoc. Cancer Res. 27:350. (Abstract no. 1390). C.R. Gardner Interactions between macrophages and mouse CNS neurons after amputation via laser microbeam surgery in monolayer cultures. University Microfilms International, Ann Arbor, Michigan. (Dissertation). C.R. Gardner and G.W. Gross Macrophages enhance the probability of neuronal survival after amputation of dendrites. Soc. Neurosci. Abstr. 11:1300. (Abstract no ). J.H. Lucas, G.W. Gross, C.R. Gardner, and J.B. Kirkpatrick Neuronal cell death after dendrite amputation. Soc. Neurosci. Abstr. 11:256. (Abstract no. 75.2). C.R. Gardner, M.H. Hightower, and G.W. Gross Macrophage attack on stressed and injured neurons in culture. Soc. Neurosci. Abstr. 10:887. (Abstract no ). C.R. Gardner and G.W. Gross Interactions between macrophages and mouse CNS monolayer cultures after neurite amputation via laser microbeam surgery. Soc. Neurosci. Abstr. 9:594. (Abstract no. 14

15 172.11). 15